Using OpenCyto to detect primary populations, and then phenograph to hunt for novel populations
- Load OpenCyto wsp
- get bool indicator for T cells
- subset FCS
- phenograph
- t-SNE
- plot OpenCyto and phenograph
Analysis
Used these markers
| HLA-DR |
| CD3 |
| CD19 |
| CD14 |
| CD16 |
| CD20 |
| CD11c |
| CD123 |
| CD56 |
TSNE results

TSNE results, colored by Phenograph clusters detected

TSNE results, colored by manual gates

TSNE results, colored by manual gates

TSNE results, colored by Phenograph clusters detected
