Using OpenCyto to detect primary populations, and then phenograph to hunt for novel populations

  1. Load OpenCyto wsp
  2. get bool indicator for T cells
  3. subset FCS
  4. phenograph
  5. t-SNE
  6. plot OpenCyto and phenograph

Analysis

Used these markers

markersDNM
HLA-DR
CD3
CD19
CD14
CD16
CD20
CD11c
CD123
CD56

TSNE results

TSNE results, colored by Phenograph clusters detected

TSNE results, colored by manual gates

TSNE results, colored by manual gates

TSNE results, colored by Phenograph clusters detected